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“The spectacle is capital accumulated to the point where it becomes image.”
Eugene Koonin, and Dr. Christoph Adami. For the full reviews, see the Reviewers' Comments section. It can be used instead of many existing specialized measurement approaches.
For example, one of the next generation sequencing techniques, RNA-seq, has replaced microarrays in nearly all application areas. Microarrays detect gene expression levels based on known probes.
By contrast, RNA-seq does not have this limitation. It can measure expression levels in any genomic region. In addition, it also provides information about SNPs and the location of splicing junctions [ 1 - 3 ].
However, one disadvantage of RNA-seq is that its sequence data can be biased by library construction.
For example, mapped read counts can over or underestimate true RNA abundances based on a variety of library construction steps, such as reverse transcription, adaptor ligation, or amplification [ 4 - 7 ].
In a comparison of multiple library preparation methods for RNA-seq, all methods introduced their own bias and showed different expression patterns from the same sample [ 8 ].
We identified the step in library preparation that caused the most pronounced bias and outlined alternative preparation techniques that can virtually eliminate this concern. They have their own library preparation methods, and other companies also developed library construction methods for the sequencers.
In previous studies, random primers or oligo dT primers were used for reverse transcription of whole transcriptome library preparation [ 5 , 10 , 11 ]. In this study, we compared different whole transcriptome libraries to each other as well as to a library specific to the human GABAB1 gene.
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